Peptide Reconstitution Guide: Step-by-Step Instructions for Safe Handling

Proper peptide reconstitution is essential to maintain stability, sterility, and effectiveness for research applications. At Proto Peptide, we provide lab-grade peptides and this guide will walk you through the correct process to prepare them safely.

1. What is Peptide Reconstitution?

Reconstitution is the process of dissolving lyophilized (freeze-dried) peptide powder into a suitable solvent such as bacteriostatic water. Correct reconstitution ensures the peptide remains stable and sterile for use in research studies.

2. What You’ll Need

Before starting, make sure you have:

Tip: Always work in a clean, controlled environment to maintain sterility.

3. Preparing Your Workspace and Vial

  1. Set up a clean, disinfected workspace before handling peptides
  2. Wash your hands or wear gloves
  3. Check the vial lid: Each peptide vial comes with a tamper-proof, sterile lid. Once removed, it cannot be replaced.

  1. Disinfect the top: Use an alcohol wipe to clean the vial’s rubber stopper before inserting the needle.
  2. Handle carefully: Avoid touching the needle or vial top to prevent contamination.

4. Adding the Bacteriostatic water

  1. Determine the required volume using our Reconstitution Calculator, which takes into account the peptide amount and desired concentration.
  2. Inject the bacteriostatic water slowly into the vial, aiming for the glass wall instead of directly on the powder. This prevents foaming or damage.

Tip: Our calculator allows you to adjust the bacteriostatic water amount between 1-3ml (1-3 full syringes if you are using a 1ml needle). The option of how much you want to dilute is up to you; however, for best results, aim to inject 10–30 diluted units on the syringe. Using less bacteriostatic water creates a more concentrated solution, which can be harder to measure accurately in the syringe. On the other hand, diluting with too much water increases the injection volume, which may cause a small lump at the injection site. Read our Subcutaneous Injection Guide for more information on proper injection methods.

5. How to roll/swirl your vial

Put the vial between your hands and gently roll it back and forth until the solution is dissolved. The liquid should be completely clear.

Storage After Reconstitution

  • Short-term: Keep the vial in the refrigerator (2–8°C / 36–46°F).
  • Long-term: For extended storage, freeze the vial at -20°C (-4°F) or lower.

Tip: Read our Peptide Storage Guide to help you store your peptides safely and maintain their integrity.

Why Proper Reconstitution Matters

  • Maintains peptide sterility
  • Prevents loss of potency
  • Ensures accurate dosing for research purposes
  • Reduces risk of contamination or degradation

Summary Checklist

✅ Use Hospira bacteriostatic water only
✅ Clean vial top with alcohol wipes before every use
✅ Follow Reconstitution Calculator for accurate volume
✅ Inject solvent slowly against the vial wall
✅ Refrigerate after reconstitution
✅ Label vials clearly